The Estimation of AAV Capsid Tier with Mass Photometry

January 24, 2024 1 min read

The Estimation of AAV Capsid Tier with Mass Photometry

AAV capsid titer is a key analytical attribute of AAV samples

When it comes to AAV sample quality, the total concentration of AAV capsid in a sample, or capsid tier is a critical factor that influences the aggregation and represents important information for downstream recovery and purification processes. 

On the other hand, titer of full AAVs also understood as the concentration of AAV capsids that contain the genetic material to be delivered is a crucial factor to determine the potency of a product and vital information for clinical dosing.


Why use mass photometry for AAV titer estimation?

When it comes to analysis of AAV samples and AAV titer estimation, mass photometers considered ideal tool due to the following reasons: 

  • Require very little sample volume and minimal sample preparation
  • Are benchtop instruments that require minimal operator training
  • Measurements are quick, results in <5 minutes

These strengths make mass photometry an efficient and easy technique to implement in environments that require repeated AAV capsid titer measurement, such as process development.


Systems for Research: Your Guide to Mass Photometry in Canada

Systems for Research is the exclusive representative for Refeyn in Canada. We work with Refeyn to make mass photometry instruments available to our customers all across Canada.

Mass photometry is a revolutionary new way to analyze molecules. It enables the accurate mass measurement of single molecules in solution, in their native state and without the need for labels. This approach opens up new possibilities for bioanalytics and research into the functions of biomolecules.