Citifluor provides a range of antifadent mounting media which greatly reduces the fading of the fluorescence from FITC, rhodamine, Hoechst, Alexa, Green Fluorescent Protein, cyanine dyes, Texas Red, and phycoerythins. A range of antifadent mountants are available in either glycerol based or glycerol free solutions. Citifluor mountant can either be permanent (hardening) or not (non-hardening) depending on your labeling requirements.
The use of fluorescent labels and markers is now widespread and immuno-fluorescence, which relies on the use of antibodies tagged with labels such as fluorescein, is a well-established technique.
A common problem associated with the microscopical examination of these materials is that the illumination used for stimulating the fluorescence also causes degradation of the label and this causes fading of fluorescence. This also is a problem when trying to visualize materials having a low level of labeling. The photofading of labeled materials can be retarded by the use of the Citifluor mountants.
Antifadent (AF) Solutions
The antifadent solutions AF-1, AF-2 and AF-3 were specifically developed for use with FITC polyclonal antibodies but their use is now far more widespread. They show improved signal preservation with many other fluorochromes including rhodamines and DAPI. AF4 contains the antifadent propyl gallate, which is particularly suitable for use with DAPI and Alexa dye-stained materials, as well as FITC-labelled materials.
AF-1 - Glycerol-phosphate buffered solution containing antifadent for use with labeled tissue and dead cells. Available in two sizes.
Gycerol based solutionsAF-1, Glycerol-PBS, with antifadent designed for antibodies labeled with FITC, also prevents photobleaching of DAPI, rhodamines, Hoechst, Alexa, cyanine dues, Texas Red, and GFP
F, T - SDS (46KB PDF)
AF-2, Glycerol with an antifadent optically transparent from 300 to 750nm, stops photobleaching of DAPI, rhodamines, Hoechst, Alexa, cyanine dues, Texas Red, and GFP
I - SDS (110KB PDF)
AF-4, Glycerol with an antifade reagent n-propyl gallate, reduces photobleaching of rhodamine and FITC, optically transparent from 400 to 750nm
AF-200 Glycerol solution containing a non-basic antifadent, high viscosity and designed for use with specimens labeled with DAPI, CY and Hoechst dye
I - SDS (315KB PDF)
AF-300 Glycerol solution of an amine-based antifadent, high viscosity and designed for use with a wide range of fluorochromes
I - SDS (111KB PDF)
Glycerol-based solutions with high refractive index (~1.52)CFM1 Glycerol-PBS solution used for transmission and epifluorescence microscopy
CFM2 Glycerol-tris amine buffered solution, pH 8.5
I - SDS (109KB PDF)
CFM3 Glycerol-based solution that contains an antifadent that isn’t an amine
I - SDS (111KB PDF)
Glycerol free solutionsAF-3 PBS solution containing an amine-based antifadent for live cell imaging
AF-100 PBS solution with amine-based antifadent, 5ml
NOTE: can be used with 19497
I - SDS (47KB PDF)
UVM1 methacrylate-based solution on exposure to light hardens to clear film. Totally water miscible (ratio of UVM1 to AF100 10:1)
I - MSDS (69KB PDF)
UVM2 methacrylate based solution containing an anti-bleaching agent, which on exposure to light hardens to give clear film. It is totally water miscible
I - MSDS (90KB PDF)
Citifluor Solid Mountant Kit – 25ml PVA, PBS solution with anti-fadent, 30ml
Components are mixed, applied to specimen and allowed to dry to a clear film
I - SDS for Citifluor AF100 Antifadent Solution, 25ml (47KB PDF)
U - SDS for Citifluor CFPVOH Solution, 5ml (44KB PDF)
Contains anti-fading solution. Suitable for preventing fading of dyes such as DAPI which have the tendency to disperse in glycerol based mounting solutions.