Chien Grids

for Transmission Electron Microscopy

1. Used to pick up many individual sections or ribbons and to transfer them onto another grid
2. It can also be formvar coated as a receiving grid for examination of a large number of sections.
3. When formvar coated, it will support 2 or even 3 parallel ribbons of serial sections, with no obscuring grid bars.
4. Hole size 50% greater than the largest slot grid.
5. There are two slits in the rim area which allow it to be easily bent with forceps to make a tab, while keeping the remainder of the grid surface flat prior to picking up ribbons.

References: Chien K, Van de Velde R, Heusser R, 1985. Simultaneous ultramicrotomy of multiple areas and examination of ribbons on one new grid. Proc 43rd Annual Meeting, Elec Micr Soc Amer, G W Bailey, ed, San Francisco Press, 460.
Galey FR, Nilsson SEG, 1966. A new method for transferring sections from the liquid surface of the trough through staining solutions to the supporting film of a grid. J Ultrastruct Res, 14, 405-410.

1. Chien One-hole Grids, 2.375mm, 3.0mm O.D., Copper, 100/vial