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22309
General Use: Immunohistochemistry
Surfaces: Glass
ready-to-use hydrophobic barrier pens
The Super PAP, Liquid Blocker Pens, are now called the A-PAP Pen (Advanced PAP Pen).
PAP Pens are used for immunohistochemical applications. The hydrophobic properties allow the user to draw barriers on the slide, in any pattern, to confine the flow of reagents to a defined area. A minimum of valuable antiserum will be used. Visible marking is light green/blue and can be removed with Xylene.
For immunohistochemistry, the PAP Pen should be applied after deparaffinization.
Directions:
Deparaffinize tissue sections and hydrate to water.
Wipe away excess liquid around the section on the glass slide with tissue paper.
Encircle the tissue section or draw lines on both sides of the section and let dry (10-15 seconds). Soak slides in PBS.
SDS (92KB PDF)
PELCO®Technical Notes, Super PAP Pen, Mini PAP Pen Liquid Blocker (PDF 87KB)
22309 - A-PAP Pen Liquid Blocker, regular size, approximately 16.5ml, tip size 5mm
22311 - A-PAP Pen Liquid Blocker, mini size, approximately 7.5ml, tip size 2mm